Gene description for VPS28
Gene name	VPS28 subunit of ESCRT-I
Gene symbol	VPS28
Other names/aliases	-
Species	Homo sapiens
Experiment description of studies that identified VPS28 in Embryonic kidney cells
1 Experiment ID 925 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 25483805    Organism Homo sapiens Experiment description Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Authors Vyas N, Walvekar A, Tate D, Lakshmanan V, Bansal D, Lo Cicero A, Raposo G, Palakodeti D, Dhawan J. Journal name Sci Rep Publication year 2014 Sample Embryonic kidney cells Sample name HEK293T - P150 - Fraction 1.12 g/mL Isolation/purification methods Differential centrifugation Ultracentrifugation Density gradient centrifugation Flotation density 1.09-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140018: EV-METRIC:56%, 56%
2 Experiment ID 1408 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36706192    Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q. Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
3 Experiment ID 1413 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV Small EVs Identification method Mass spectrometry PubMed ID 36706192    Organism Homo sapiens Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles Authors Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q. Journal name Sci Adv Publication year 2023 Sample Embryonic kidney cells Sample name HEK293T - Fraction 5 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density 1.151 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
4 Experiment ID 1671 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
5 Experiment ID 1671 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry EV-TRACK -
6 Experiment ID 1672 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -
7 Experiment ID 1672 Identified molecule Protein Extracellular vesicle type Exosomes MISEV Small EVs Identification method Mass spectrometry PubMed ID 34108659    Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry EV-TRACK -